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Other potential technologies to identify the most viable oocytes:
polar body biopsy, spindle imaging, Zona pellucida
birefringence
Markus Montag, Ph.D., Prof.
University of Heidelberg
Polar body biopsy and array-CGH: the impact of chromosomal disorders
Test - DNA
CONTROL- DNA
COT 1-DNA
Software-based
evaluation
Maternal age and aneuploidy rates
• Geraedts J, Montag M, Magli C et al. Polar body array CGH for prediction of the status of the corresponding oocyte. I. Clinical results. Hum. Reprod. 2011, 26(11):3173-80.
• Magli C, Montag M, Köster M et al. Polar body array CGH for prediction of the status of the corresponding oocyte. I. Technical aspects. Hum. Reprod. 2011, 26(11):3181-5
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Age < 30 Age 31 - 340 Age > 40
Aneuploidy rate in stimulated cycles
Spindle imaging by polarization microscopy: visualizing the invisible
Is this a metaphase-II-oocyte?
Spindle dynamics is physiological
Spindle bridge
MI -> MII
75-90 min MII
40-60 min
Zona imaging by polarization microscopy: a parameter of cytoplasmic maturity
Zonaimage – Cytoplasmic Maturity Zona = product of the oocyte Optimal maturation = good structured zona
Zona imaging: clinical use
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HZB/HZB HZB/LZB LZB/LZB Kontrolle
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% 20 50 65 68 cycles
LZB/LZB versus HZB/LZB and HZB/HZB
* P < 0.005
# P < 0.025
Pregnancy rate
Implantation rate
Zona imaging: the molecular background
Recommended reading: Assou et al., Hum Reprod Update 2011
Pregnancy No pregnancy
Seven significantly differential expressed genes
in cumulus cells
Assidi, Montag, van der Ven, Sirard; JARG, 2011
The viable oocyte
• Primary target: oocyte maturity / follicular background
• Methodology: Polarization microscopy
o Spindle imaging for meiotic maturity
o Zona imaging for developmental competence
• Secondary target: oocyte aneuploidy
• Methodology:
o Polar body biopsy (PB1+2) and array-CGH
• Oocyte viability assessment is a valuable add-on for subsequent selection of the most implantation-competent embryo
Further reading
• Ebner T, Balaban B, Moser M et al. Automatic user-independent zona pellucida imaging at the oocyte stage allows for the prediction of preimplantation development. Fertil Steril, 2010;94:913–20.
• Madaschi C, Aoki T, de Almeida Ferreira Braga DP et al. Zona pellucida birefringence score and meiotic spindle visualization in relation to embryo development and ICSI outcomes. Reprod Biomed Online 2009;18:681–6.
• Madaschi C, Aoki T, de Almeida Ferreira Braga DP et al. Zona pellucida birefringence score and meiotic spindle visualization in relation to embryo development and ICSI outcomes. Reprod Biomed Online 2009;18:681–6.
• Montag M, Köster M, van der Ven K, van der Ven H. Gamete competence assessment by polarization microscopy. Hum Reprod Update, 2011;17:654-66.
