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VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO

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Page 1: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 2: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 3: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 4: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO

pGEMR-T Easy

• Dient zur leichten Klonierungvon PCR-Produkten

Page 5: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 6: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 7: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 8: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 9: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 10: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 11: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 12: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO

pBluescript, ein klassisches Phagemid

Page 13: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 14: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 15: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 16: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO

Substitutionsvektoren

Linker Arm „Stuffer“ Rechter Arm

IntegratLigation

Page 17: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 18: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO
Page 19: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO

Sal Bam Eco Eco Bam Sal

Verdau mit Sal I + Eco

Sal Bam Eco Eco Bam Sal

Sal

Sal

Eco

Eco

Sal

Sal

Fällen mit Isopropanol

Eco

Eco

Sal

Sal

Integrat

Linker Arm Rechter Arm„Stuffer“

Page 20: VL Gentechnologie4 06 - uni-mainz.de · TOPO Blunt-end PCR product TOPO TOPO 3 phosphate zero Blunt. TOPCP vector Figure 1 - TOPO TA Cloning. Topolsomerase recognition sites TOPO

Lambda Zap:Moderne Vektoren Mit Phagen und Plasmid-eigenschaften