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CHARACTERIZATION OF
MEMBRANE BIOFOULING INNANOFILTRATION
PROCESS OF
WASTEWATER TREATMENT
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Objective
Introduction
Methodology
Result and discussion
Conclusion and perspective
Table of content
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Identify and characterize the structure, morphologyand
composition of the membrane biofouling layer onnanofiltration (NF)
process of wastewater treatment
Objective
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Biofouling process involves adhesion and growth ofmicroorganisms
on the membrane surface, thus it damagesthe membrane
Introduction
Biofilm formation involves accumulation of microorganismsat a
phase transition interface (solid-liquid, gas-liquid,
orliquid-liquid)
Biofouling is hard to control
Bacteria accumulate on membrane by 2 processes:
attachment(adhesion,adsorption) and growth (multiplication)
Biofouling is hard to control
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Experimental setup
Operational conditions
Bacterial community composition analysis
Fourier transformed infrared (FTIR) spectra
Methodology
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NF membrane biofouling was synthesized by mimickingsecondary
effluents and prefiltered secondary effluents.
Experimental setup
DISMOUNTING
NF SYSTEM (polyamide
tubular membrane,filtration area of 120 cm2)
SEGMENTING (10 slices) andANALYZING (SEM, DGGE)
DILUTION and FTIRspectroscopy
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Test different flow and pressure conditions (constanttemperature
= 25oC)to set the system operation pointfor biofouling
experiments
Biofouling experiments utilized synthetic effluents as
well as real secondary effluents for characterizationof the
biofouling layer
Operational conditions
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Measurement of membrane
Fourier transformed infrared spectra
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RESULT AND DISCUSSION
- Initial Flavobacterium concentration = 105 CFU/ml
1. NF biofouling by glucose based synthetic effluents and amodel
bacterium
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RESULT AND DISCUSSION (cont)
2. NF biofouling of a starch-based synthetic effluents and
amodel bacterium
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RESULT AND DISCUSSION (cont)
3. NF experiments with MF-prefiltered secondary effluents
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RESULT AND DISCUSSION (cont)
4. Identification of bacterial population and characterization
ofbiofouling layer
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RESULT AND DISCUSSION (cont)
5. FTIR spectra of NF experiments
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Both glucose (approx. 80% rejection) and starch(approx. 90%)
interacted similarly with the system inspite of the significant
difference in their molecularweight
Biofouling appears to be the predominant form offouling and the
size and composition of biofilmpopulation seem to be
convergent.
Biofouling layer over the membrane reduced flux andimproved
brine rejection.
CONCLUSION AND PERSPECTIVE
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FTIR analysis of membranes applied to synthetic effluent
dis- played an accumulation of polysaccharide, amide
andaliphatic structures in the middle segment of themembrane (in a
tubular con- figuration). This is possiblyreflected by an enhanced
biofilm development in themiddle segments of the NF membrane.
FTIR analysis of membranes applied to synthetic effluent
dis- played an accumulation of polysaccharide, amide
andaliphatic structures in the middle segment of themembrane (in a
tubular con- figuration). This is possiblyreflected by an enhanced
biofilm development in themiddle segments of the NF membrane.
CONCLUSION AND PERSPECTIVE
(cont)
