Pipettenspitzen_EN Fcha Tecnica

8/10/2019 Pipettenspitzen_EN Fcha Tecnica

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H I G H - T E C H D I S P O S A B L E

Pipette tips and filter tips are

manufactured by BRAND in

a cleanroom under the most

modern production condi-

tions, and are automaticallyrack packed and packaged.

Standard- and Ultra Low Re-tention pipette tips

Graduation for a quick volumecheck

All tips and filter tips up to1000 l are free of DNA(< 40 fg), RNase (< 8.6 fg),

endotoxins (< 1 pg) and ATP (< 1 fg)

Autoclavable at 121 C (2 bar),acc. DIN EN 285

Environmentally friendly pack-aging systems

CE-marked according to IVD-Directive 98/79 EC

The tip systemfrom BRAND

TipBox - TipRack - TipStack


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Colored tip-trays with sidepanel labeling

The contents of the box arealways clearly visible.

Usable with a partiallyloaded multichannel pipette

The rimless tip-tray borderenables problem-free loadingof individual pipette tips with

multichannel instruments.

Opening and closingwith one hand

The clamping mechanismholds the tip-tray securely

in the box.

Dual functionhinged and push-on lid

Simply rotate 180 tochange closure method.

Transparent window

For better all-roundvisual inspection.

Low the TipBox is optimized forpipette tips and filter tips up to

300 l, high TipBox up to 1000l. Stackable.

All siz es avail able in 96-uni tformat

No deflection duringtip loading

The tip-tray is fabricatedfrom especially rigid PP.

TipStack

rder to guarantee the highlity of our products, fromufacturing through packag-

and all the way to the labora-, BRAND has also optimizedpackaging options to com-with the new requirements.n the primary packaging fornew systems, such as the

T outer packaging for theTipRacks (refill units) and

he cleanest t ip technologythe spacers for the new Tip-Stacks, are produced undercleanroom conditions, whichguarantees the high purity ofthe products.

All pipette tips a nd fi lter tips u pto 1000 l are now free fromDNA, RNases, endotoxins and

ATP, indep enden t of the pack-aging formats. Sterile tips andpackaging are manufacturedexclusively under BIO-CERT quality certification.

TipBox

TheTipBox- TipRack - TipStack TM-System


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BRAND disposable items for the life sciences are produced usingthe most advanced cleanroom techniques in one of the worlds larg-est cleanrooms for laboratory disposable items.The ongoing cleanroom monitoring includes continuous measure-ments of air particulates, positive air pressure, air exchange rate,room temperature, and the relative humidity, among other things.This ensures that the actual parameters can immediately bechecked against the nominal values. Deviations are detected im-mediately, and suitable countermeasures can be taken before thelimit values are exceeded.

The high-precision control ofenvironmental conditions pro-vides a very high degree ofstability in the correspondingparameters, especially the roomtemperature. This uniformity, to-gether with quality testing of thefinal product by batch, guaran-tees the consistently high qualityin the life science products fromBRAND.

For the production of disposableitems, Class 8, 7, and 5 clean-rooms are available, all validat-ed according to ISO 14 644-1.Compliance with ISO 14 644-1is certified by external, indepen-dent auditors.

Pipette tips and filter tips arethe most frequently used dis-posables in the laboratory. Asprocessing methods have be-

come increasingly sensitive, therequirements for these dispos-able items have changed signif-icantly over the years. Starting

with the raw materials, here PP,to the tools used and the qual-ity tests carried out, a lot of pa-rameters need to be consideredto meet the highest standardsboth in research and standard-ized applications.

Consistently high quality

For the manufacture of pipette tips and filter tips, BRAND exclu-sively uses raw materials that are free from the additives di(2-hy-droxyethyl)methyldodecylammonium (DiHEMDA) and 9-octadece-namide (oleamide). Both of these additives are frequently foundin PP granules, and can interfere with biological tests, leading tospurious results. Only highly polished tooling equipment is used, sothat no parting agents or demolding aids are needed.

Cleanroom manufacture


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TipRack Compared to the filled TipBoxes,the amount of waste with thenew refill units is reduced by over20%. All pipette tips and filtertips up to 1000 l are free ofDNA, RNases, endotoxins and

ATP. TipRacks in BIO-CERT

quality are sterile according toISO 11 137 and the AAMI guide-lines, a SAL of 10-6 is obtained.

TipStack TM

A tip tower containing 5 filled tip-trays and a TipBox constitute thenew, space-saving refill systemfor 20 l, 200 l and 1000 l tips.Tightly sealing spacers preventthe tips from getting stuck to-

gether, and ensure them to befree from DNA, RNases, endo-toxins and ATP.The sterile TipStacks (BIO-CERT quality) are supplied witha transfer aid for contamination-free use in a previously sterilizedTipBox.

Sterile handling

All components are recy-clable

Reduced amount of waste

Sterilizable and reusableTipBox

High purity of the pipettetips and filter tips

Space-saving design

Transfer aid

TipRacks

Press the long sides of the trans-fer aid together, and continuepressing them together while

withdrawing the tip- tray.Ensure that the holding strapsfor the transfer aid are correctlypositioned.

Insert the filled tip-tray perpen-dicularly from above into the previ-ously sterilized TipBox until it locksinto place.

Remove the transfer aid from themounting plate. Finished all

without tip contact!

These racks are supplied with atransfer aid that enables simple,contamination-free transfer intoa previously sterilized TipBox. Alltip-trays are printed on one side

with information on the contents.

TipStack


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0 . 5

- 2 0

l

1

- 5 0

l

2 - 2

0 0

l

0 . 1

- 2 0

l

0 . 5

- 1 0

l

2

- 2 0

l

0 . 1

- 1

l

1

- 2 0

l

0.1 - 20 l0.1 - 1 l (Fil ter)The nano-cap tip was spe-cially developed for the nano-liter range and hence is idealfor molecular biological ap-plications such as PCR. It is37 mm long, and features acapillary rise that is even visibleto the naked eye at 0.1 l. Thecapillary part of the tip con-veniently fills gel wells for gelelectrophoresis systems frommost manufacturers. Suitablefor pipettes up to 20 l. Theracked tips are colorless andplaced into a grey-colored tiptray.

2 - 200 l2 - 20 l (Fil ter)

A tested and proven thin- walled tip. Lighter in weight,50 mm long and can be usedfor virtually all pipettes withyellow color code. Graduationat 20 l and 100 l for a quickvolume check. Bulk tips areyellow colored. The racked tipsare colorless and placed into ayellow-colored tip tray.

0.5 - 20 l0.5 - 10 l (Fil ter)The slender design and 46 mmlength allow pipetting in micro-tubes and microplates withouttouching the walls. Graduationat 2 l and 10 l for quickvolume check. The racked tipsare colorless and placed into agrey-colored tip tray.

1 - 50 l1 - 20 l (Fil ter)With a length of 50 mm, the tipis ideal for working down to thebottom of narrow containers.Graduation at 2.5, 10, 25 and50 l for quick volume check.The racked tips are colorlessand placed into a grey-coloredtip tray.

TipBox,steri le and non-steri le

PP. With hinged lid/push-on lid.Two different sizes. Stackableand repeatedly autoclavableat 121 C (2 bar), acc. DINEN 285.

Bulk packed in bags,non-steri le

All tips and f ilter tips are produ-ced under supervised state-of-the-art clean-room conditionsand automatically shrink-wrap-ped in reclosable bags andpackaged in cardboard boxes.The batch number is printed onevery bag.

Racked (TipRack),steri le and non-steri le

For TipBox. Refill unit, pro-tected in an environmentallycompatible packaging of re-cyclable PET. Sterile TipRacksare supplied with a transfer aidso that the rack can be put intoa previously autoclaved box

without hand contact.

Pipette t ips and Filter TipsSizes and Description

Package Types

NEW!

NEW!


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5 - 3

0 0

l

5 0 - 1

0 0 0

l

0 . 5

- 5 m

l

1 - 1

0 m

l

5 - 1

0 0

l

5 - 2

0 0

l

5 0 - 1

0 0 0

l

5 - 300 l5 - 100/200 l (Fil ter)The thin-walled tip is suitablefor reverse pipetting and plate

washing. It is 53 mm long andcan also be used for pipettes

with yellow color code. It is par-ticularly suited for working withmultichannel pipettes. Gradua-tion at 50, 100 and 300 l forquick volume check. The rackedtips are colorless and placedinto a green-colored tip tray.

50 - 1000 l50 - 1000 l (Fil ter)Proven thin-walled tip for rou-tine laboratory and researchpipetting. Its length is 70 mm.Graduation at 250, 500 and1000 l for a quick volumecheck. Bulk tips are blue col-ored. The racked tips are col-orless and placed into a blue-colored tip tray.

0.5 - 5 mlParticularly slender shape, at160 mm length and approx.9.6 mm diameter! This al-lows pipetting even from narrowvolume measuring equipment

such as volumetric flasks withNS 12/21. Suitable for mi-croliter pipette Transferpette and Thermo Fisher ScientificFINNPIPETTE.

1 - 10 ml156.5 mm length and ap-prox. 15 mm diameter! Idealfor working with the microliterpipette Transferpette S 10 ml.Compatible with Eppendorf

systems.

TipStack ,s teri le and non-steri le

Space-saving, environmentallycompatible refill system forTipBox. 5 racks, each having96 tips incl. 1 TipBox. SterileTipStacks are supplied with atransfer aid so that the rackcan be put into a previouslyautoclaved box without handcontact. Each packaging unitcontains 2 TipStacks.

TipBox 5/10 ml,non-steri le

The 5 ml and 10 ml tips are onlyavailable as a racked tip versionin this specially correspondingTipBox.

NEW!

NEW!

Sterile pipette tips fromBRAND are manufacturedunder certified BIO-CERTquality.


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Quantity Pack of non-sterileCat. No.

sterileCat. No.

bulk 2 000 2 bags, 1000 each 7320 02 bulk XXL 10 000 10 bags, 1000 each 7320 22 racked 960 10 TipRacks, 96 each 7321 02 7321 22TipBox 480 5 boxes, 96 each 7322 02 TipBox sterile 960 10 boxes, 96 each 7322 22TipStack

Pipette Tips

0.1 - 20 l

1 - 50 l

2 - 200 l


sterileCat. No.

bulk 2 000 2 bags, 1000 each

7320 06

bulk XXL 10 000 10 bags, 1000 each 7320 26 racked 960 10 TipRacks, 96 each 7321 06 7321 26TipBox 480 5 boxes, 96 each 7322 06 TipBox sterile 960 10 boxes, 96 each 7322 26TipStack


sterileCat. No.

bulk 1 000 1 bag, 1000 each 7320 08 bulk XXL 10 000 10 bags, 1000 each 7320 28 racked 960 10 TipRacks, 96 each 7321 08 7321 28TipBox 480 5 boxes, 96 each 7322 08 TipBox sterile 960 10 boxes, 96 each 7322 28TipStack 960 2 x 5 racks, 96 each 7322 48 7322 68


sterileCat. No.

bulk 2 000 2 bags, 1000 each 7320 04 bulk XXL 10 000 10 bags, 1000 each 7320 24 racked 960 10 TipRacks, 96 each 7321 04 7321 24TipBox 480 5 boxes, 96 each 7322 04 TipBox sterile 960 10 boxes, 96 each 7322 24TipStack 960 2 x 5 racks, 96 each 7322 44 7322 64

0.5 - 20 l

Pipette tips, 0.1 - 20 l

Pipette tips, 0.5 - 20 l

Pipette tips, 1 - 50 l

Pipette tips, 2 - 200 l (bulk tips are yellow colored)

TipBox, with tip-tray,empty

PP. Stackable. Pack of 1.

Model Cat. No.

up to 20 l 7329 90

for 200 l 7329 92

for 300 l 7329 94

for 1000 l 7329 96


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5 - 300 l

50 - 1000 l


sterileCat. No.

bulk 1 000 1 bag, 1000 each 7320 10 bulk XXL 10 000 10 bags, 1000 each 7320 30 racked 960 10 TipRacks, 96 each 7321 10 7321 30TipBox 480 5 boxes, 96 each 7322 10 TipBox sterile 960 10 boxes, 96 each 7322 30TipStack


sterileCat. No.

bulk 1 000 2 bags, 500 each 7320 12 bulk XXL 5 000 10 bags, 500 each 7320 32 racked 960 10 TipRacks, 96 each 7321 12 7321 32TipBox 480 5 boxes, 96 each 7322 12 TipBox sterile 960 10 boxes, 96 each 7322 32TipStack 960 2 x 5 racks, 96 each 7322 52 7322 72

0.5 - 5 ml

1 - 10 ml


sterileCat. No.

bulk 200 2 bags, 100 each 7026 03 bulk XXL 1000 10 bags, 100 each 7026 04 racked TipBox 10 ml 18 1 box, 18 each 7026 08 TipStack

Pipette tips, 5 - 300 l

Pipette tips, 50 - 1000 l (bulk tips are blue colored)

Pipette tips, 0.5 - 5 ml

Pipette tips, 1 - 10 ml


sterileCat. No.

bulk 200 1 bag, 200 each

7025 95

bulk XXL 1000 5 bags, 200 each 7026 00 racked TipBox 5 ml 28 1 box, 28 each 7026 05 TipStack

bulk racked TipBox TipStack TipBox 5 ml


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Filter TipsNon-self-sealing filter tips from BRAND havea PE filter that is free from chemical addi-tives. Permeability is controlled by the combi-nation of pore size and filter length, so that noaerosols can reach the pipette shaft. Thesefilters function with consistent reliability. On

the other hand, liquids can pass very slow-ly should they accidentally contact the filter.


sterileCat. No.

bulk 960 1 bag, 960 each 7325 04 racked 960 10 TipRacks, 96 each 7326 04 7326 24TipBox 480 5 boxes, 96 each 7327 04 TipBox sterile 960 10 boxes, 96 each 7327 24

0.5 - 10 l


sterileCat. No.


1 - 20 l

Filter tips, 0.5 - 10 l

Filter tips, 1 - 20 l


sterileCat. No.


0.1 - 1 l

Since the filter does not swell as in the caseof self-sealing filter tips, the sample can berecovered from the filter by simply actuatingthe pipettes blow-out function, or by cen-trifugation if necessary. This is clearly an im-portant advantage of non-self-sealing filters,

especially when working with valuable sam-ples. As an added advantage, the absenceof filter additives protects samples from con-tamination.

Filter tips, 0.1 - 1 l

P i p e

t t e

t i p

F i l t e r

t i p


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2 - 20 l

5 - 100 l

5 - 200 l

50 - 1000 l


sterileCat. No.



sterileCat. No.

bulk 960 1 bag, 960 each 7325 10 racked 960 10 TipRacks, 96 each 7326 10 7326 30

TipBox 480 5 boxes, 96 each 7327 10 TipBox sterile 960 10 boxes, 96 each 7327 30


sterileCat. No.

bulk 960 1 bag, 960 each 7325 12

racked 960 10 TipRacks, 96 each 7326 12 7326 32TipBox 480 5 boxes, 96 each 7327 12 TipBox sterile 960 10 boxes, 96 each 7327 32


sterileCat. No.






bulk racked TipBox


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sterileCat. No.

TipBox 480 5 boxes, 96 each 7323 02 TipBox sterile 960 10 boxes, 96 each 7323 22TipStack

0.1 - 20 l


sterileCat. No.

TipBox 480 5 boxes, 96 each 7323 04 TipBox sterile 960 10 boxes, 96 each 7323 24TipStack 960 2 x 5 racks, 96 each 7323 44 7323 64

0.5 - 20 l

ULR pipette tips, 0.1 - 20 l

ULR pipette tips, 0.5 - 20 l

Ultra Low Retention Pipette TipsThe surfaces of the Ultra Low Retentiontips are produced through a special, pat-ented physicochemical process. The homo-geneous, defect-free surface thus producedhas extremely low surface tension over50% less than PTFE (see table). This sig-

nificantly reduces sample loss and providessubstantially higher reproducibility when

working with critical media.

Surface Surface tension

BRAND PP Ultra Low Retention 9 mN/mPTFE 19 mN/m

Silicone 21.5 mN/mUntreated PP 30 mN/mWater 72 mN/m

Ideal for biological samples that containdetergents such as TRITON X-100,SDS, Tween etc.

No additives that can be leached out!No siliconization of the surface!

High chemical resistance. Ideal for wor-king with solvents.

The tips can be autoclaved at 121 C(2 bar) without damaging the materialproperties.

U l t r a

L o w

R e

t e n

t i o n p

i p e

t t e

t i p

S t a n d a r

d p

i p e

t t e

t i p


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1 - 50 l

2 - 200 l


sterileCat. No.



sterileCat. No.


ULR pipette tips, 1 - 50 l


5 - 300 l

50 - 1000 l


sterileCat. No.



sterileCat. No.




TipBox TipStack


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ULR filter tips, 0.1 - 1 l


sterileCat. No.

TipBox 480 5 boxes, 96 each 7328 04

TipBox sterile 960 10 boxes, 96 each 7328 24

0.5 - 10 l


sterileCat. No.


1 - 20 l

ULR filter tips, 0.5 - 10 l

ULR filter tips, 1 - 20 l


sterileCat. No.


0.1 - 1 l

2 - 20 l

5 - 100 l


sterileCat. No.



sterileCat. No.




Ultra Low Retention Fil ter Tips


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1

2

3

0.5

1.5

4

2.5

3.5

4.5

1.84

1.2

2.37

1.3

2.31

1.4

4.58

0.1

1.8

0.023

2.5

0.04

2.37

0.06

0.5

0.04

0.1

0.05

0.15

0.2

0.15

0.05

5 - 200 l

50 - 1000 l


sterileCat. No.




% R

e s i d u a l q u a n

t i t y

Standard and Ultra Low Retention pipette tips from BRAND

Volume 200 l, compared with various media and subsequent photometric analysis and conversion.

BRAND Ultra Low Retention pipette tips

% R

e s i d u a l q u a n

t i t y

PCR master mixBSA 1% SDS Methanol100%

Food coloring(green)

TRITON X-100

Isopropanol60%

n-Decane DMSO

Comparison chart


sterileCat. No.

TipBox 480 5 boxes, 96 each 7328 14

TipBox sterile 960 10 boxes, 96 each 7328 34

BRAND standard pipette tips

TipBox


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T r a n s f e r p e t t e


e l e

c t r o n i c


- 8 /

- 1 2


- 8 /

- 1 2 e l e

c t r o n i c


S


S - 8 / - 1 2

Pipette Tips and Filter Tips in Standardand Ultra Low Retention Quality

Pipette and filter tips from BRAND are tested for BRAND pipettes and most

of the pipette types of GILSON

, Thermo Fisher Scientific FINNPIPETTE

,Eppendorf and BIOHIT/sartorius.The 5 ml tip is exclusively tested for BRAND pipettes and Thermo FisherScientific FINNPIPETTE. The 10 ml tip is tested for BRAND and Eppendorf.

Optimum results are achieved in combination with BRAND microliter pipettes.BRAND quality tips seat perfectly for precise analyses.

The right Pipette Tip

P i

p e t t e t i p s

F i l t er

t i p s

V o

l um er a n

g e

Transferpette single channelNominal volume

Transferpette multichannelNominal volume

1

l

2 . 5

l

5

l

1 0

l

1 0

l * *

2 0

l

2 0

l * *

2 5

l

5 0

l

1 0 0

l

2 0 0

l

2 0 0

l * * *

2 5 0

l

5 0 0

l

1 0 0 0

l

2 m

l

5 m

l

1 0 m

l

1 0

l

2 0

l

2 5

l

5 0

l

1 0 0

l

2 0 0

l

3 0 0

l

0.1 - 20 l

0.5 - 20 l

1 - 50 l

2 - 200 l*

5 - 300 l

50 - 1000 l*

0.5 - 5 ml

1 - 10 ml0.1 - 1 l

0.5 - 10 l

1 - 20 l

2 - 20 l

5 - 100 l

5 - 200 l

50 - 1000 l = Tip volume less than pipette's nominal v olume* Bulk tips are yellow or blue colored, racked tips are colorless in a yellow-colored or blue-colored tip tray ** Transferpette with yellow color code

*** Transferpette

with blue color code

Note: Pipette shafts can be subject to modification. The fit depends on the manufacturer, pipette type,serial number, and date of manufacture, among other things. We recommend checking the fit of thetips using the free sample bag.


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DN A ATP

BIO-CERT

A corresponding certi ficate is included in delivery.

Tips and filter tips up to 1000 l are available in sterile quality.They are manufactured under certified BIO-CERT quality.

Steril i ty BRAND products are sterilized with beta radiationaccording to ISO 11 137 and the AAMI Guidelines. The radiationdose is at least 12.1 kGy.

An SAL (sterility assurance level) of 10-6 is guaranteed, meaning nomore than one part in 1 x 106 is contaminated! This level of sterilitycomplies with the requirements of USP 29 and the Ph.Eur.

DNA and RNases RNases are ubiquitous and extremelystable. To protect the RNA molecules from undergoing enzymaticdegradation, one must be absolutely certain that plastic items arefree from RNases.Products from BRAND are free from DNA (< 4 x 10-14 g/tip = 40 fg),to avoid false positive results, e.g., in PCR*, and free from RNases(< 8.6 x 10 -15 g/tip = 8.6 fg), to make it possible to work with RNA.

Endotoxins Endotoxins refer to the components pres-ent in the outer membranes of Gram-negative bacteria. Thesecomponents are lipopolysaccharides that are released when cellsare destroyed. The concentration of endotoxins in products fromBRAND is determined by the kinetic-turbidimetric limulus ame-bocyte lysate (LAL) test. The detection limit is 0.01 EU/ml. Thiscorresponds to an endotoxin concentration of < 1 x 10-12 g/tip(1 pg/tip). Freedom from endotoxins is required in pharmaceuticalmanufacture and cell culture.

AT P ATP is an energy-r ich, transportable molecule. It is an indi-cator for living cells. Products from BRAND are free of ATP (ATPconcentration < 1 x 10-15 g/tip = 1 fg) and thus are especially suit-able for luminescence measurements, e.g., those employed in thehygiene monitoring field according to the HACCP concept.

free of DNA and RNases, Endotoxins and ATP All tips and filter t ips up to 1000 l are

steri le

BIO-CERT products are sterile, free from human DNA,RNases, endotoxins and ATP.


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BRAND GMBH + CO KG P.O. Box 11 55 97861 Wertheim GermanyTel.: +49 9342 808-0 Fax: +49 9342 808-98000 E-Mail: [email protected] Internet: www.brand.de

92

PrnednGermany

131113

Transferpette, BIO-CERT and BRAND are trademarks of BRAND GMBH + CO KG, Germany.Other reproduced brands are the property of the respective owner.

Our technical literature is intended to inform and advise our customers. However, the validity of general empirical values, and ofresults obtained under test conditions, for specific applications depends on many factors beyond our control. Please appreciate,therefore, that no claims can be derived from our advice. The user is responsible for checking the appropriateness of the productfor any particular application.

Subject to technical modification without notice. Errors excepted.

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Pipettenspitzen_EN Fcha Tecnica