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Quality creates value
www.dornier-ltf.com
®
The future in pipetting
Quality creates value
www.dornier-ltf.com 600 x 515 x 470 mm
Hardware Overview
®
Quality creates value
www.dornier-ltf.com
Deck Layout Small outside measurements but much space inside
- 9 full size SBS plate positions
- 7 further accessory SBS plate and rack
positions
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Quality creates value
www.dornier-ltf.com
Deck PositionsEnough space to set up 384 different samples without adding tips
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Quality creates value
www.dornier-ltf.com
Pipetting Heads Pipetting heads can be exchanged within seconds
• Pipetting Head 200, 1-channel with an optimal pipetting volume of 1 - 180 µl• Pipetting Head 1000, 1-channel with a maximum volume of 1ml
Two heads are available
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Quality creates value
www.dornier-ltf.com
Pipetting Heads
Pipetting small volumes with 50µl tips
The height of the PIRO® allows for primary tubes to be used with 1ml tips
®
Quality creates value
www.dornier-ltf.com
Level SensingThe PIRO® level sensor relies on pressure detection allowing
the robot to automatically detect when the pipetting tip is reaching the liquid level
Advantages
• Ensures correct pipetting volumes
• Use of non-conductive tips, cost reduction
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Quality creates value
Examples to a variety of plates and racks on the PIRO®
PIRO® Plate Racks
TR-7H; 7 x 5ml tubes
TR-11A x 0.5/0.6 ml
TR-8F; 8 x 2/ 0.5 mlTR-8C; 8 x 1.5 ml tubes
TR-8V; 8 x 2 ml tubes
TR-28A; 28 x 0.2 ml
PIRO® Tube Racks
96-well Rack (P100318) Waste channel (P100322)
Tip Rack (P100317)Waste Rack (P100321)
Plate Rack-1 (P100319) Plate Rack-2 (P100417)
®
Quality creates value
www.dornier-ltf.com
Waste Handling
wastechanneloutside
waste boxinside
waste bagwaste bin waste drawer
Waste can be discarded inside or outside the PIRO®
®
Quality creates value
www.dornier-ltf.com
Waste HandlingWaste can be discarded inside or outside the PIRO®
®
Quality creates value
www.dornier-ltf.com
Specials for a PIRO®
…as well for me?
error free pipetting? precise in repeats? secure into 384-well plates? pipetting from a data base? permanent supervision of all pipetting steps?
complete chain of data logging?
…simple and fast by a PIRO!
®
Quality creates value
www.dornier-ltf.com
Softwaredrag & drop
do it within minutes
Piro.lnk
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Quality creates value
www.dornier-ltf.com
Moving PlatesPlates can be moved even after functions have been assigned
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Quality creates value
www.dornier-ltf.com
A simple set of sample information given by a text file like…
Position Name for PCR 1 for PCR 2 5.A1 Probe 1 1 (yes) 1(yes)
…and translated into a pipetting operation determined by the template…
… is imported by the PIRO® software as a task list…
Intelligent Pipetting
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Quality creates value
www.dornier-ltf.com
Import Data – Sample Import
From CSV files
• Barcode reading
• Intelligent pipetting
®
Quality creates value
www.dornier-ltf.com
Barcode ReadingBarcode reading can be done for samples and plates
®
Quality creates value
www.dornier-ltf.com
Export DataPIRO® run data can be exported to various different file formats by simply using the function ‘Reaction export’. Save the file in the appropriate file format and import the information into the software of the desired real time instrument.
Well Name Id Conc. Reaction 1
5.D1 aa 111 0.5 1
5.D2 bb 222 0.5 1
5.D3 cc 333 0.5 1
5.D4 dd 444 0.5 1
5.D5 ee 555 0.5 1
5.D6 ff 666 0.5 1
5.D7 gg 777 0.5 1
5.D8 hh 888 0.5 1
®
Quality creates value
www.dornier-ltf.com
precísion&
accuracy
®
Quality creates value
www.dornier-ltf.com
Checker Board TestingThe PIRO® pipetting robot has extensively been tested using “Checker Board” setups. A checker board test is where positive and negative samples are run next to each other to verify no Cross-contamination
For such tests, various plate formats and real time instruments have been used, a few examples are shown below
®
Quality creates value
www.dornier-ltf.com
Checker Board Testing
Tests have been performed using Reagent from Congen
Positive and negatives samples could clearly be separated showing no cross contamination between samples and NTC
Rep. Ct Std.: 0.13Rep. Ct: 20.65Dev. Rep. Ct (95% CI) [20.60, 20.69]
36 positive samples 36 NTCs
®
Quality creates value
www.dornier-ltf.com
Testing small VolumesEven lower volumes down to 0.5µl have been tested also showing that a high performance can be achieved with the PIRO. Tests below show replicates using
12µl + 3µl (black), 14µl + 1µl (green), and 14.5µl + 0.5µl (red).
Tests have been performed using 2 x SensiMix from Bioline
12µl + 3µl (8x)Rep. Ct Std.: 0.08Rep. Ct: 19.0114µl + 1µl (30x)Rep. Ct Std.: 0.11Rep. Ct: 20.7214.5µl+0.5µl (30x)Rep. Ct Std.: 0.14Rep. Ct: 21.21
Result: Consistent results with low variation have been achieved over many runs
®
Quality creates value
www.dornier-ltf.com
Results Pipetting
The PIRO® accurately pipettes replicates with high
precision and accuracy.
The PIRO® can be used in the multi-dispensing mode
delivering accurate results and therefore saving time
and speeding up the pipetting operations
®
Quality creates value
www.dornier-ltf.com
Special Hardware Features
Documentation
Special Software Features
- UV decontamination procedure (optional)- HEPA filter unit (optional)
- Sample normalization (also with imported data)- axial (re-)calibration of all plates and racks by the user
- pre- and post-run reports available as PDF files- all operational steps and exceptions / warnings are recorded- CSV export of reactions created by a run is possible
®
Quality creates value
www.dornier-ltf.com
Safety
Auto switch to pause the PIRO® when opening the lid
Info, warnings, error, and reminders when setting up a run
Lid to protect the user from harm, but also reagents
Level sensing and mixing options for accurate pipetting
Templates to make sure the run is identical to previous runs
Check list before starting a run
Pre- and post run reports, password protectable documents
®
Quality creates value
www.dornier-ltf.com
Low maintenance, low service costs
No pipetting errors, less repeats
Cost Saving
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Easy to use import and export functions
A perfect mate for BioRad CFX, Roche Light Cycler, RotorGene and other brands as well
Piro.lnk
Quality creates value
2012-11www.dornier-ltf.com
®
Thank you!
