Dette projekt finansieres af midler fra Den Europæiske Fond for Regionaludvikling. | Dieses Projekt wird gefördert mit Mitteln des Europäischen Fonds für regionale Entwicklung.

Faculty of TechnologyDepartment of Chemical Engineering, Biotechnology and Environmental Technology

PhD student Signe PtakAssociate prof. Knud V. Christensen

Prof. WSR Xavier Fretté

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Optimizing fucoidan yield from Baltic Fucus brown algae genera

© Picture: Zenthoefer

Agenda

1. Fucoidan

2. Applications for fucoidan

3. Background for project and extraction procedure

4. Preliminary results

5. Future experiments

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Fucoidan

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• Sulfated polysaccharide (wide range of molecular weights)

• Found in brown algae and brown seaweedsuch as mozuku, kombu, bladderwrack, wakame and hijiki

Purpose and characteristics

• Protection against water-borne pathogens and other environmental challenges (e.g. osmotic stress).

• Fucoidans are polysaccharides characterized by a complex molecular structure and varying degrees of sulphation and acetylation.

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Structure

• Extraction, species, plant maturity, origin....

• Fucose backbone

5H. R. Fletcher et al. Algal Research 22 (2017) 79-86

Applications for fucoidan

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Medicine – Prevention of AMD

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But also!

Medicine

• Tissue engineering (promotes bone healing)

• Anti-cancer

• Anti-bacterial

Cosmetics

• Anti-wrinkle

• Whitening

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Our research

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Extraction, Extraction, Extraction, ……• Optimizing extraction and

purification procedure of fucoidanfrom F. vesiculosus (bladderwrack)

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Yield across different genera and regions

• Extraction and purification of fucoidan from three Fucus species:

- F. vesiculosus

- F. serratus

- F. evanescens

• Harvested in Summer and Autumnof 2017.

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What affects the yield?

• Genera

• Region

• Temperature

• Solvent

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Extraction solvent

• Looking for other alternatives to using hydrochloric acid

Sulfate ester cleaving

Low sulfatefucoidan

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Sulfuric acid?

H. R. Fletcher et al. Algal Research 22 (2017) 79-86

Extraction and clean-up

• Initial de-fatting step

• Extraction

• Precipitation of

- Alginate

- Laminarin

- Crude Fucoidan

• Dialysis

• Size-Exclusion Chromatography

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Extraction and clean-up

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Microwave Assisted Extraction (MAE)

• Minimize extraction steps for full extraction

• Seaweed contain heavy metals

promotes heat formation during microwaving

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Precipitated crude fucoidan Dried crude fucoidan

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DialysisRemoval of salts and non-bound sulfate

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Preliminary Size-Exclusion Chromatography (SEC) results• Pullulan standards

P05, P10, P100, P200, P400, P800

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P05 P10 P100 P200 P400 P800 Sigma Extract

13.36min

12.78min

10.83min

10.22min

9.65min

9.14 min

8.49 min

7.90 min

Sigma: Fucoidan SLBS-0192VExtract: sulfuric acid at 120°C

Preliminary Yield Results

MAE at 80°C MAE at 100°C

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5,77

0 3,5 7

FV

FS

FE

Yield [WT%]

Fugus genus

H2SO4

H2O

5.09

0 3,5 7

FV

FS

FE

Yield [WT%]

Fucus genus

H2O

H2SO4

HCL

Future work

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Upscaling of extraction • Likely with sulfuric acid extraction

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Bioassay testing

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Contact

Department of Chemical Engineering, Biotechnology and Environmental Technology, University of Southern Denmark

Xavier Fretté

xafr@kbm.sdu.dk

Signe H. Ptak

sihp@kbm.sdu.dk

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www.fucosan.eu

”Health From the Sea”

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