Publikationen und ausgewählte Vorträge

Originalarbeiten in Fachzeitschriften mit Peer Review

1. Hofmann T, Fischer A, Meiler J, Kalkhof S Protein structure prediction guided by crosslinking constraints – a systematic evaluation of the impact of the crosslinking spacer length Methods, 2015 (15), 207-8

2. Müller SA, Pernitzsch SR, Haange S, Uetz P, von Bergen M, Sharma CM, Stefan Kalkhof S Stable isotope labeling by amino acids in cell culture based proteomics reveals differences in protein abundances between spiral and coccoid forms of the gastric pathogen Helicobacter pylori Journal of Proteomics, 2015;126:34-45

3. Ahrens VM, Kostelnik KB, Rennert R, Böhme D, Kalkhof S, Weber L, von Bergen M, Beck-Sickinger AG A Cleavable Cytolysin-Neuropeptide Y Bioconjugate Enables Specific Drug Delivery and Demonstrates Intracellular Mode of Action Journal of Controlled Release, 2015;209:170-8

4. Hofmann T, Samsonov SA, Pichert A, Lemmnitzer K, Schiller J, Huster D, Teresa Pisabarro M, von Bergen M, Kalkhof S Structural analysis of the Interleukin-8/Glycosaminoglycan interactions by amide hydrogen/deuterium exchange mass spectrometry, Methods 2015 [Epub ahead of print]

5. Kalkhof S, Dautel F, Loguercio S, Baumann S, Trump S, Jungnickel H, Otto W, Rudzok S, Potratz S, Luch A, Lehmann I, Beyer A, von Bergen M Pathway and Time-Resolved Benzo[a]pyrene Toxicity on Hepa1c1c7 Cells at Toxic and Subtoxic Exposure Journal of Proteome Research 2014 [Epub ahead of print]

6. Uhlmann, KR, Gibb, S, Kalkhof, S, Arroyo-Abad, U, Schulz, C, Hoffmann, B, Stubbins, F, Carpenter, S, Beer, M, von Bergen, M, Feltens, R Species determination of Culicoides biting midges via peptide profiling using matrix-assisted laser desorption ionization mass spectrometry, Parasites & Vectors, 2014, 7:392

7. Kalkhof, S, Förster, Y, Schmidt, J, Schulz, MC, Baumann, S, Weißflog, A, Gao, W, Hempel, U, Eckelt, U, Rammelt, S, von Bergen, M Proteomics and metabolomics for in situ monitoring of wound healing Biomed Research International 2014, 934848

8. Murugesan K, Baumann S, Wissenbach DK, Kliemt S, Kalkhof S, Otto W, Mogel I, Kohajda T, von Bergen M, Tomm JM Subtoxic and toxic concentrations of benzene and toluene induce nrf2-mediated antioxidative stress response and affect the central carbon metabolism in lung epithelial cells A549 Proteomics 2013;13:3211-3221

9. Muller SA, Findeiss S, Pernitzsch SR, Wissenbach DK, Stadler PF, Hofacker IL, von Bergen M, Kalkhof S

Identification of new protein coding sequences and signal peptidase cleavage sites of helicobacter pylori strain 26695 by proteogenomics Journal of Proteomics 2013;86:27-42

10. Mobius K, Nordsieck K, Pichert A, Samsonov SA, Thomas L, Schiller J, Kalkhof S, Pisabarro MT, Beck-Sickinger AG, Huster D Investigation of lysine side chain interactions of interleukin-8 with heparin and other glycosaminoglycans studied by a methylation-NMR approach Glycobiology 2013;23:1260-1269

11. Kliemt S, Lange C, Otto W, Hintze V, Moller S, von Bergen M, Hempel U, Kalkhof S Sulfated hyaluronan containing collagen matrices enhance cell-matrix-interaction, endocytosis, and osteogenic differentiation of human mesenchymal stromal cells Journal of Proteome Research 2013;12:378-389

12. Blumert C*, Kalkhof S*, Brocke-Heidrich K, Kohajda T, von Bergen M, Horn F Analysis of the stat3 interactome using in-situ biotinylation and SILAC Journal of Proteomics 2013;94:370-386

13. Schaks S, Kalkhof S, Krauth F, Jahn O, Sinz A Chemisches Cross-Linking und MS zur Untersuchung von Proteinkomplexen BioSpektrum 2012;17:524-527

14. Salbach J, Kliemt S, Rauner M, Rachner TD, Goettsch C, Kalkhof S, von Bergen M, Moller S, Schnabelrauch M, Hintze V, Scharnweber D, Hofbauer LC The effect of the degree of sulfation of glycosaminoglycans on osteoclast function and signaling pathways Biomaterials 2012;33:8418-8429

15. Muller SA, van der Smissen A, von Feilitzsch M, Anderegg U, Kalkhof S, von Bergen M Quantitative proteomics reveals altered expression of extracellular matrix related proteins of human primary dermal fibroblasts in response to sulfated hyaluronan and collagen applied as artificial extracellular matrix Journal of Materials Science: Materials in Medicine 2012;23:3053-3065

16. Lipstein N, Schaks S, Dimova K, Kalkhof S, Ihling C, Kolbel K, Ashery U, Rhee J, Brose N, Sinz A, Jahn O Non-conserved ca2+/calmodulin binding sites in munc13s differentially control synaptic short-term plasticity Molecular and cellular biology 2012; 32: 4628–4641

17. Gundel U, Kalkhof S, Zitzkat D, von Bergen M, Altenburger R, Kuster E Concentration-response concept in ecotoxicoproteomics: Effects of different phenanthrene concentrations to the zebrafish (danio rerio) embryo proteome Ecotoxicology and environmental safety 2012;76:11-22

18. Goettsch C, Kliemt S, Sinningen K, von Bergen M, Hofbauer LC, Kalkhof S Quantitative proteomics reveals novel functions of osteoclast-associated receptor in stat signaling and cell adhesion in human endothelial cells Journal of molecular and cellular cardiology 2012;53:829-837

19. Baumann S, Kalkhof S, Hackermüller J, Otto W, Tomm J, Wissenbach D, Müller S, Rolle-Kampczyk U, von Bergen M Requirements and perspectives for integrating metabolomics with other omics data

Current Metabolomics 2013; 1:15-27

20. Abburi R, Kalkhof S, Oehme R, Kiontke A, Birkemeyer C Artifacts in amine analysis from anodic oxidation of organic solvents upon electrospray ionization for mass spectrometry European Journal of Mass Spectrometry (Chichester, Eng) 2012;18:301-312

21. Washietl S, Findeiss S, Muller SA, Kalkhof S, von Bergen M, Hofacker IL, Stadler PF, Goldman N Rnacode: Robust discrimination of coding and noncoding regions in comparative sequence data RNA 2011;17:578-594

22. Morbt N, Tomm J, Feltens R, Mogel I, Kalkhof S, Murugesan K, Wirth H, Vogt C, Binder H, Lehmann I, von Bergen M Chlorinated benzenes cause concomitantly oxidative stress and induction of apoptotic markers in lung epithelial cells (A549) at nonacute toxic concentrations Journal of Proteome Research 2011;10:363-378

23. Goettsch C, Lauckner S, Sinningen K, Schoppet M, von Bergen M, Kalkhof S, Aikawa E, Hofbauer LC Novel role of the osteoclast-associated receptor (oscar) in oxidative stress-mediated atherogenesis Circulation 2011;124

24. Dautel F, Kalkhof S, Trump S, Michaelson J, Beyer A, Lehmann I, von Bergen M DIGE-based protein expression analysis of b[a]p-exposed hepatoma cells reveals a complex stress response including alterations in oxidative stress, cell cycle control, and cytoskeleton motility at toxic and subacute concentrations Journal of Proteome Research 2011;10:379-393

25. Dautel F, Kalkhof S, Trump S, Lehmann I, Beyer A, von Bergen M Large-scale 2-D DIGE studies-guidelines to overcome pitfalls and challenges along the experimental procedure Journal of Integrated OMICS 2011;1:170-179

26. Muller SA, Kohajda T, Findeiss S, Stadler PF, Washietl S, Kellis M, von Bergen M, Kalkhof S Optimization of parameters for coverage of low molecular weight proteins Analytical and bioanalytical chemistry 2010;398:2867-2881

27. Kalkhof S, Witte K, Ihling CH, Muller MQ, Keller MV, Haehn S, Smyth N, Paulsson M, Sinz A A novel disulfide pattern in laminin-type epidermal growth factor-like (le) modules of laminin beta 1 and gamma 1 chains Biochemistry 2010;49:8359-8366

28. Kalkhof S, Haehn S, Paulsson M, Smyth N, Meiler J, Sinz A Computational modeling of laminin n-terminal domains using sparse distance constraints from disulfide bonds and chemical cross-linking Proteins: Structure, Function, and Bioinformatics 2010;78:3409-3427

29. Gundel U, Kalkhof S, von Bergen M, Altenburger R, Kuster E Concentration-response concept in ecotoxicoproteomics: An optimised design for the detection of robust chemical stress associated protein markers: Effects of different concentrations of phenanthren to the proteome of danio rerio embryos Comparative Biochemistry and Physiology A 2010;157:S42-S43

30. Feltens R, Gorner R, Kalkhof S, Groger-Arndt H, von Bergen M Discrimination of different species from the genus drosophila by intact protein profiling using matrix-assisted laser desorption ionization mass spectrometry BMC evolutionary biology 2010;10

31. Morbt N, Mogel I, Kalkhof S, Feltens R, Roder-Stolinski C, Zheng J, Vogt C, Lehmann I, von Bergen M Proteome changes in human bronchoalveolar cells following styrene exposure indicate involvement of oxidative stress in the molecular-response mechanism Proteomics 2009;9:4920-4933

32. Manolaridis I, Mumtsidu E, Konarev P, Makhov AM, Fullerton SW, Sinz A, Kalkhof S, McGeehan JE, Cary PD, Griffith JD, Svergun D, Kneale GG, Tucker PA Structural and biophysical characterization of the proteins interacting with the herpes simplex virus 1 origin of replication Journal of Biological Chemistry 2009;284:16343-16353

33. Dimova K, Kalkhof S, Pottratz I, Ihling C, Rodriguez-Castaneda F, Liepold T, Griesinger C, Brose N, Sinz A, Jahn O Structural insights into the calmodulin-munc13 interaction obtained by cross-linking and mass spectrometry Biochemistry 2009;48:5908-5921

34. Kalkhof S, Sinz A Chances and pitfalls of chemical cross-linking with amine-reactive n-hydroxysuccinimide esters Analytical and bioanalytical chemistry 2008;392:305-312

35. Kalkhof S, Haehn S, Ihling C, Paulsson M, Smyth N, Sinz A Determination of disulfide bond patterns in laminin beta 1 chain n-terminal domains by nano-high-performance liquid chromatography/matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry Rapid Communications in Mass Spectrometry 2008;22:1933-1940

36. Schulz DM, Kalkhof S, Schmidt A, Ihling C, Stingl C, Mechtler K, Zschoernig O, Sinz A Annexin a2/p11 interaction: New insights into annexin a2 tetramer structure by chemical crosslinking, high-resolution mass spectrometry, and computational modeling Proteins: Structure Function and Bioinformatics 2007;69:254-269

37. Ihling C, Schmidt A, Kalkhof S, Schulz DM, Stingl C, Mechtler K, Haack M, Beck-Sickinger AG, Cooper DMF, Sinz A Isotope-labeled cross-linkers and fourier transform ion cyclotron resonance mass spectrometry for structural analysis of a protein/peptide complex Journal of the American Society for Mass Spectrometry 2006;17:1100-1113

38. Sinz A, Kalkhof S, Ihling C Mapping protein interfaces by a trifunctional cross-linker combined with maldi-tof and esi-fticr mass spectrometry Journal of the American Society for Mass Spectrometry 2005;16:1921-1931

39. Schmidt A, Kalkhof S, Ihling C, Cooper DM, Sinz A Mapping protein interfaces by chemical cross-linking and fourier transform ion cyclotron resonance mass spectrometry: Application to a calmodulin / adenylyl cyclase 8 peptide complex

European Journal of Mass Spectrometry (Chichester, Eng) 2005;11:525-534

40. Kalkhof S, Ihling C, Mechtler K, Sinz A Chemical cross-linking and high-performance fourier transform ion cyclotron resonance mass spectrometry for protein interaction analysis: Application to a calmodulin/target peptide complex Analytical chemistry 2005;77:495-503

41. Berger K, Wissmann D, Ihling C, Kalkhof S, Beck-Sickinger A, Sinz A, Paschke R, Fuhrer D Quantitative proteome analysis in benign thyroid nodular disease using the fluorescent ruthenium ii tris(bathophenanthroline disulfonate) stain Molecular and cellular endocrinology 2004;227:21-30

Ausgewählte Vorträge

Nagel M, Schaarschmidt J, Meiler J, Kalkhof S, Paschke R „Chemical crosslinking of TSHR-ECD” Deutsche Gesellschaft für Massenspektrometrie, 46 Diskussionstagung, Wuppertal 2015

Kalkhof S, Nagel M, Schaarschmidt J, Meiler J, Paschke R „Identification and structural analysis of protein complexes by mass spectrometry based techniques” 4th Scientific Leipzig/Nashville Symposium, Nashville, USA, 2013

Goettsch C, Kliemt S, Sinningen K, von Bergen M, Hofbauer LC, Kalkhof, S „Quantitative proteomics reveals novel functions of osteoclast-associated receptor in STAT signaling and cell adhesion in human endothelial cells”, Deutsche Gesellschaft für Massenspektrometrie, 46 Diskussionstagung, Gießen 2012

Kalkhof S, Blumert C, von Bergen M, and Horn F “Analysis of the STAT3 interactome using in-situ biotinylation and SILAC” 10th Annual World Congress of the Human Proteomics Association, Geneva 2011

Kalkhof, S, Goettsch, C, Kliemt, S, Sinningen, K, von Bergen, M, and Hofbauer, L C, “OSCAR (Osteoclast-associated immunoglobulin-like receptor) – new functions revealed by quantitative proteomics” International Symposium Frontiers in biomaterial science, Dresden 2011

Kalkhof, S, “Structure analysis of proteins and protein complexes using mass spectrometry based methods” Einladungsvortrag, Universität Wien, Wien 2011

Kalkhof, S, Haehn, S, Ihling, C, Stingl, C, Mechtler, K, Paulsson, M, Smyth, N and Sinz, A, „Disulfide Bond Patterns of Laminin Beta1 and Gamma1 N-Terminal Constructs Analyzed by ESI-FTICR-MS and MALDI-TOF/TOF-MS”, Deutsche Gesellschaft für Massenspektrometrie, 41 Diskussionstagung, Gießen 2008

Kalkhof, S, Haehn, S, Paulsson, M, Smyth, N, Meiler, J, and Sinz, A, “Computational modeling of laminin N-terminal domains using sparse distance constraints from disulfide bonds and chemical cross-linking” Seminar of the Center of Structural Biology, Nashville 2006