Speicher

DNA(Gen)ATG.CTC..

Botschaft

mRNA

AUG.CUC…

Produkt Metabolit A

Protein(Enzym) Metabolit BMet.Leu...

Informationsfluss Stofffluss

5000 4000 4000 2000 Genom Transkriptom Proteom Metabolom

Abb.1

RRegulator

144 aa

PPromotor

T PTransport

Peri-plasma116 aa

TransportMembrane

91 aa

AMercury-

Reductase561 aa

BMercury-

Lyase212 aa

Hg(II)

Abb.2

Secretion Degradation

RG Promotor Operator TF Struc. DNA

mRNA

EnzymeMetabolite P

Transcription

TranslationInhibition

Degradation

Apo-repressor

Repressor

Feedbackrepression

Operon

Abb. 3

idijiFiii

i mRNAkcccTODdt

dmRNA ..,..,)( 21

Transkription

isijiii

i EkcccmRNAdtdE

...,...., 21

Translation

...),,...,( 121 jjii ccccErij Metabolische Flüsse

GEN

PRODUKTdcd t

r cjij

i

n

j

Abb.4

40 45 50 55 60 65 70 750

2

4

6

t3

t2

Phase IIPhase IC

B

A

Tryp

toph

an

Time

6

7

8

9 Phase IIPhase It1

Enzy

me

0,00,30,60,9

mRN

A

Abb. 5

Abb. 6

MALSYTSJ

AroBAroCAroDAroIAroE

AroAAroF

AspBCitZ

ASPOBUT

THR

ILE

TKT2 TAL

RPE

GLUC

3PG

G3P

F6P

PEP

PYR AcCoA

G6P

E4P

X5P

SER

GLY

Rib5P

R5P

CHOR PHEN

TYR

PHE

VAL

GLK

PGI

PFKFBATPI

PGMENO

PYK

ZWF GntZ

RPI

PdhA PdhBAcAcCoA

MmgA

GlyA

TKT

AroA

IlvA

SerB SerC SerA

GLU

GLN

MDH

OAA CIT

AKGMAL

SUCCFUMA

CitBCitC

OdhBSucCSucDFumA

GlnA

GAPPGK

PtsI

PycA

SdhA

GltB

YclMASDHOMKhsE

IlvCIlvDIlvE

HOThPPOIVAIlvE IlvB

IlvCIlvD

ThrC

LEU

LeuALeuB

LeuCDIlvE

PdhC PdhD

AspB

AspBTyrA

PheA

CYS

CysECysK

MmgB

3-HBCoA

PHB

PHBSynthase

PTA

Acetyl-P

AckA

Acetate

Abb. 7

GenomeAnnotation

EnzymesEC 1.1.1.3EC 1.1.1.6

EC 1.1.1.17

Reversibility information

Connectionmatrix

C00013-C00009C00022-C00900C01083-C00267

Reactions Homoserine + NAD+ = Aspartate-Glycerol + NAD+ = Glycerone +Mannitol 1-P + NAD+ = F6P + N

…………………

Reaction database

(e.g. Ligand, Brenda

Nonenzymatic reactions

Ma & Zeng (2003) Bioinformatics. 19:1423-30

Reaction

Metabolite

Abb. 8

MethionineCysteine

sulfur

One carbon, folate, glycine, serine, threonine

TCA

Phenylalanine tyrosine tryptophan

Urea cycle, nitrogen, glutamate, arginine, proline, pyrimidine

Fatty acidValine, leucine

isoleucine

Pentose phosphate fructose, mannose galactose

Purine

Purinede novo

Glycerolipid, glycosphingolipid

Methane

Lysine

EC numbers: 434Metabolites: 1172

Glycolysis, pyruvate

Abb. 9

.

rpoE

fis

soxR

cspA

rpoS

phoB

crp

ihf

argP

cspE

rpoNdnaA

hnscytR

Global regulators

Regulatory modules

Abb. 10

Nahrung = 350 Signale

Gen mRNA Protein

A

B

Osmolarität pH-Wert Temperatur

Sauerstoff

Antibiotika

Druck

Licht

Erdanziehungskraft

Gift/Gefahr

Botschaften andererBakterien Scherstress

Nährstoff-limitierung

Abb. 11

Abb. 12

Abb, 13

Abb. 14

Shaking Flask

1µm

Couette flow bioreactor

= 0.028 s-1 = 1482 s-1

Abb. 15

Keimung2

50 µm8 Stunden

Pellet

Wachstum4

Sporen SporenpaketePrimäreAggregation

1

50 µm2 Stunden

SekundäreAggregation

3

100 µm12 Stunden

Abb. 16

Pelle

tkon

zent

ratio

n [1

/L]

Pelle

tdur

chm

esse

r [m

m]

0,1

1,0

10,0

104

105

106

0,050 0,060 0,070 0,080 0,090 0,100spezifischer Leistungseintrag [W/kg]

Akt

ivitä

t [µK

at/L

Rea

ktor

volu

men

]

0

0,2

0,4

0,6

0,8

1,0

1,2

1,4

1,6

1,8

0

20

40

60

80

100

120

140

160

180

Akt

ivitä

t [µK

at/m

L Pe

lletv

olum

en]t = 80 h

Abb.17

Genom-Sequenzen

Proteom(Proteine)

Transkriptom(mRNA)

gezielte Exp.Metabolic Eng.

Zellphysiologie Kinetik

Metabolom Fluxom

Bioprozesse& Bioprodukte

EnvironomVerfahrenstechnik

metabolisches Netzwerk

genetisches Netzwerk

Abb. 18