biomol-Mikroskop Elektron

8/16/2019 biomol-Mikroskop Elektron

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Mikroskop Elektron

Sutiman B. SumitroGuru Besar Biologi Sel

Universitas Brawijaya

Understanding Size

1 metre

Understanding Size

10 centimetres


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Understanding Size

1 centimetre

Understanding Size

100 micrometres

Understanding Size

10 micrometres


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Understanding Size1 micrometre

Understanding Size100 nanometres

Understanding Size

10 nanometres


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Understanding Size1 nanometre

Tahun 1973 Tahun 2013

Mikroskop Elektron Transmisi

The electron microscope uses electrostatic and

electromagnetic lenses

Electron microscopes are used to investigate the ultrastructure of

a wide range of biological and inorganic specimens

Industrially, the electron microscope is often used for quality

control and failure analysis.

Modern electron microscopes produce electron micrographs,

using specialized digital cameras or frame grabbers to capture

the image

Resolustion achived to 50 piko meter (pm) resolutionandmagnifications of up to about 10,000,000x

https://en.wikipedia.org/wiki/Electrostatic_lenshttps://en.wikipedia.org/wiki/Electromagnetismhttps://en.wikipedia.org/wiki/Ultrastructurehttps://en.wikipedia.org/wiki/Micrographhttps://en.wikipedia.org/wiki/Frame_grabberhttps://en.wikipedia.org/wiki/Frame_grabberhttps://en.wikipedia.org/wiki/Micrographhttps://en.wikipedia.org/wiki/Ultrastructurehttps://en.wikipedia.org/wiki/Electromagnetismhttps://en.wikipedia.org/wiki/Electrostatic_lens


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Resolusi (Daya Pisah)

1 nm 100 nm

Newtonian

Gravitation,

speed

Space and time

Measurable

dimension,

weigh, andspeed

Modern

Physics

Quantum

Physics

Atom andSubatomic,

particless

Time and

space less

Nano Science


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Sample preparation

A sample of cells (black) stained withosmium tetroxide and uranyl acetateembedded in epoxy resin (amber) ready for

sectioning.

Tissue Sectioning

A diamond knife blade used for cutting

ultrathin sections (typically 70 to 350 nmfor transmission electron microscopy.


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Imaging methods

Contrast formation

Contrast formation in the TEM depends greatly on the mode of operation.Complex imaging techniques, which utilize the unique ability to change lens

strength or to deactivate a lens, allow for many operating modes

Bright field The most common mode o f operation for a TEM is the bright

field imaging mode

Diffraction contrast known as a dark-field image.

Electron energy loss This normally results in chromatic aberration –

however this effect can, fo r example, be used to generate an image which

provides information on elemental composition, based upon the atomic

transition during electron-electron interaction.

Phase contrast Crystal structure can also be investigated by High-

Resolution Transmission Electron Microscopy (HRTEM)

Diffraction This image provides the investigator with information about thespace group symmetries in the crystal and the crystal's orientation

TEM can be modified into a

Scanning TransmissionElectron Microscope (STEM)

by the addition of a system

that rasters the beam acrossthe sample to form the image

Scaning

Transmision

Electon

Microscope

STEM)

A three-dimensiona l T EM image of a parapoxavirus

http://en.wikipedia.org/wiki/Space_grouphttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://localhost/var/www/apps/conversion/tmp/scratch_7/TEM-parapoxvirus-tomograph.ogv.480p.webmhttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Scanning_transmission_electron_microscopehttp://en.wikipedia.org/wiki/Space_group


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Scaning Electron Microscope

M. von Ardenne's f irst SEM

Images of Scanning Electron

Microscope

1 micrometre

10 micrometres

100 nanometres


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SEM

NORMAL

OSTEOPOROSIS

XRD PATTERN

XRD PATTERN

http://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/NORMAL%20BONE.cmdfhttp://localhost/var/www/apps/conversion/tmp/scratch_7/OSTEOPOROSIS.cmdf


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1

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Average of crystal size in osteoporosis

0

50

100

150

200

( n m )

HA-Synthetic Normal Osteoporosis

Skull Bone Cortical Bone

Crystal Size

Data (15) D (15)

Crystal size

(nm)

1 HAp std 1899,15 189,92

2 S-16 263,31 26,33

3 S-15 197,43 19,74

4 S-2 263,85 26,39

5 S-4 716,83 71,68

6 M5 790,46 79,05

7 Bone Skull 88,99 8,90

8 Normal

Cortical Bone

350,73 35,07

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Atomic distribution pattern in osteoporosis

Ca P

Cu Zn

Al Fe K Mg S Ti


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Atomic distribution pattern in normal

Ca K Ti

Cu Zn Fe

Al P Mg S

Comparation of mapping atom (SEM-EDAX)

Pattern Osteoporosis Normal Periodic

1 Ca, P Ca, K, Ti IV

2 Cu, Zn Cu, Zn, Fe IV

3 Al, Fe, K, Mg, S, Ti Al, P, Mg, S III

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http://localhost/var/www/apps/conversion/Periodic%20Table.lnkhttp://localhost/var/www/apps/conversion/Periodic%20Table.lnk

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biomol-Mikroskop Elektron