»Redaktionssysteme«

oyen.de | 2011

KW So. 26/08/12

im Detail Mögliche Wege von Daten, über Layout zu unterschiedlichen KW So. 26/08/12

EckdatenGründung oyen.de2001

Schwerpunkt als Berater, Trainer & Medienprogrammierer Lösungsfindung für Publishing Herausforderungen

Schwerpunkte als Autor Berichterstattung über Trends, Software und Technologien die das systemgestützte Publizieren in die Ausgabekanäle Print, Web und mobile Endgeräte beeinflussen [Multi Channel Publishing]

0/3

Veröffentlichungen (Auszug 2011-2007)

2011☐ Umfrage: Trends 2011 • Publishing & Kommunikation • Medienkanäle & Formate | Oyen Dez 2010 - Jan 2011

2010☐ Multichannel Publishing für Kataloge, Zeitungen und Zeitschriften (PP Nov/Dez 2010) ☐ Multi Format Publishing | iPad Publishing - Von Print zur digitalen Seitengestaltung (PP Nov/Dez 2010) ☐ Multi Format Publishing | Quark XPress für Blio - Print Content multimedial anreichern (PP Sep/Okt 2010) ☐ Multi Format Publishing | Content goes Online: Adobe Indesign als Editor von Webseiten benutzen (PP Jul/Aug 2010) ☐ Definition Multi Format Publishing (Jun 2010)☐ Geschäftsmodelle und Grundregeln beim Aufbereiten von Print-Content für E-Book-Titel (PP Mai/Jun 2010) ☐ Der Weg zum Neugeschäft: Mit »altem« Content auf mobilen Endgeräten (PP Mar/Apr 2010) ☐ [ ] checklist publishing | market overview HTML | PDF (Feb 2010) ☐ Informations-Management: Multi-Channel-Publishing für Print, Web, Devices zweiter Teil - Ausgabe und Trends bei Publishing-Systemen (PP Jan/Feb 2010)

2009☐ Informations-Management: Multi-Channel-Publishing für Print, Web, Devices erster Teil - Input und Verarbeitung mit Publishing-Systemen (PP Nov/Dez 2009)☐ Database Publishing mit Adobe InDesign CS4 und QuarkXPress 8 - ein Testbericht (PP Jul/Aug 2009)☐ Vom Publishing 1.0 zum Publishing 3.0 – die Katalogproduktion geht neue Wege [FR US] (The Dynamic Publisher Mar 2009)☐ E-Book - Technologie, Geräte u. Datenformate (PP Jan/Feb 2009)☐ Multi-Channel-Publishing: Systeme für die Redaktion von morgen (PP Jan/Feb 2009)☐ 2009 Die Zukunft ist da - über den Umgang mit Medienbrüchen (Jan 2009)☐ Medienbruch - ein deutsches (Un)wort und seine Bedeutung im Publishing Umfeld (Jan 2009) 2008☐ Vier Säulen | Database Publishing Integration (Okt 2008)☐ DIN 16511 Korrekturzeichnen für PDF Dokumente (Sep 2008)☐ TCS | Single Source / MultiChannel Publishing (Mai 2008) (technische kommunikation tk 3/08) 2007 ☐ Tools für technische Dokumentation (Nov 2007)☐ Print-to-Web | Vorstellung des Redaktionssystemes K4 | PDF (Aug 2007)☐ Adobe CS3 | Paket für mediale Grenzgänger (Jul 2007) (technische kommunikation tk 4/07)☐ Catalog Trends 2007 Outtake ( Jun 2007)☐ Verteiltes Arbeiten im Netz mit Adobe InDesign (Mai 2007)☐ Das Adobe Creative Suite 3 Universum / Grafik (Mai 2007)☐ Flash und PDF - wenn Dokumente mobil und Inhalte interaktiv werden (Jan 2007)

... kompletter Auszug -> http://oyen.de/press

KW So. 26/08/12

Zeitstrahl

ePaper & mobile Devices

Einführung DBP 1. InDesign Konferenz

webasierte Werbemit-telprodu-ktion via W2P

DBP InBetween / Einrichtung / Training & Markteinführung

2010 2009 2007 2005 2004 2001 1999 1994 1986

Print2iPad / digital

Publishing

Katalog-

trends Print goes Web /

Multi Channel

Publishing / Collabo-

ration

1. DBP

Marktüber-sicht Adobe

InDesign / QuarkX-

Press

CD-ROM Kataloge auf PDF-

Basis / BluePrint

Search&Order

DTP mit

Aldus Pagemaker

0/4

KW So. 26/08/12

PRINT WEB

DEVICES

PIM

PublishingSysteme

Gestalten & Veredeln

AusgabeDatenbereit-stellung

CRM

ResearchMaFo

MRM

Medien Information Manager Vehikel

MAM

360° Publishing Blick

0/5

E-Mailing

Newsletter

Magalog

Geschäftsberichte

Pressemitteilungen

KundenmagazineBücher

Zeitung Magazin

Handbuch

Katalog /Preisliste

Handbuch

BroschürenAnzeige

POSPOS

eBook

mobile

Spielconsolen

smartphone

Kiosk*

Katalog /Preisliste

eBook

mobile

Spiel-konsolen

TV Radio

smartphone

Bücher

Zeitung Magazin Geschäfts-berichte

Presse-mitteilungen

Kunden-magazine

BroschürenAnzeigeVerpackung

Kiosk*

Handbuch

E-Mailing

Webshop

Produktfinder

SocialMedia

Newsletter

POS

Magalog

PRINT WEB

DEVICES

Werbemittel

Marken

Kommunikation

Prod

ukt

Information & Unterhaltung

Verla

g

PR / Unternehmens -

Kom

mun

ikat

ion

E-Mailing

Newsletter

E-Mailing

Newsletter

mobileiPadTablet

KW So. 26/08/12

Redaktionssysteme

1) Der Weg von Daten über Layout zur Ausgabe

2) Systemansätze und Überschneidungen

3) flexibles Layout ./. automatisiertes Layout

4) Komponenten

5) Zusammenarbeit

0/6

Folien 0/1 - 0/6 oyen.de 1/7 - 1/9 Der Weg von Daten über Layout zur Ausgabe2/10 - 2/15 Programmiervorlagen & Publishing Prozesse3/16 - 3/35 Umsetzungen (Auszug) 2002 - 20114/37 - 4/38 Teamaufstellung5/39 - 5/40 Methode & Vorgehen

KW So. 26/08/12

Der Weg von Daten über Layout zur Ausgabe

1

KW So. 26/08/12

Ausgabe (Kanal)

� Print

Katalog

Magalog

Magazin

Anzeige

Newsletter

Broschüre

Preisliste

Geschäftsberichte

POS

Verpackung

Dokumentation

� Web

WebShop

Portal

Konfigurator

Newsletter

� mobileEndgeräte

iPad

Tablet

Smartphone

eBook

KIOSK

Daten

� Bereitstellung

� Abgleichen

� Sammeln

� Filtern

� Aufbereiten

� Vervollständigen

Layout

� Programmierung Vorlagen

Beispiele

Zuweisungen

Ergebnis

Seiten / Objekte Layout

Daten / Asset Verknüpfung

Ausgabeformate Print

Web

mobile Endgeräte

RegelwerkeSeitenaufbau / Umbuch

Objektaufbau / Verwendung

Stilvorgaben Absatz

Zeichen

Grep

� Abstimmung /Freigabe

Korrekturworkflow

Statusüberwachung

Remote Publishing / web2Print

� AdaptionFormate

Seiten

Sprachen

Kanal

� ÜbernahmeDaten

Import CSV / XML

http:// Request

ODBC

� ZusammenstellungDokumenten für Ausgabe

� Print

� Web

� mobileEndgeräte

� AktualisierungInhalte

bidirektional

� Konvertierung Seitenformate

JPEG

PDF Print

Web

Office

PNG

� Umsetzung

Seitenaufbau (Print)

Aufbereitung mit Daten

Verknüpfen mit Daten und Assets aus Dateisystem

� Export Inhalte

RTF

XML

� Erstellung TOC / Index / Querverweise

Seitenverweise

Sprungziele Web/URL

Neuer Knoten

� Multimediales Anreichern

SlideShow

Audio / Video Player

Interaktion- Animation - Anwendung- E-Mail / URL- Schaltflächen / Navigation- Formulare

Web-Inhalte

Popup-Windows

scrollbare Bereiche

Reflow-Inhalte (ePub /iPad) oyen.de | 2011

Daten > Layout > Ausgabe

1/8

KW So. 26/08/12

Ausgabe (Kanal)

� Print

Katalog

Magalog

Magazin

Anzeige

Newsletter

Broschüre

Preisliste

Geschäftsberichte

POS

Verpackung

Dokumentation

� Web

WebShop

Portal

Konfigurator

Newsletter

� mobileEndgeräte

iPad

Tablet

Smartphone

eBook

KIOSK

Daten

� Bereitstellung

� Abgleichen

� Sammeln

� Filtern

� Aufbereiten

� Vervollständigen

Layout

� Programmierung Vorlagen

� Abstimmung /Freigabe

� AdaptionFormate

� ÜbernahmeDaten

� ZusammenstellungDokumenten für Ausgabe

� Print

� Web

� mobileEndgeräte

� AktualisierungInhalte

� Konvertierung Seitenformate

� Umsetzung

� Export Inhalte

� Erstellung TOC / Index / Querverweise

� Multimediales Anreichern

Ausgabe (Kanal)

� Print

Katalog

Magalog

Magazin

Anzeige

Newsletter

Broschüre

Preisliste

Geschäftsberichte

POS

Verpackung

Dokumentation

� Web

WebShop

Portal

Konfigurator

Newsletter

� mobileEndgeräte

iPad

Tablet

Smartphone

eBook

KIOSK

Daten

� Bereitstellung

� Abgleichen

� Sammeln

� Filtern

� Aufbereiten

� Vervollständigen

Layout

� Programmierung Vorlagen

Beispiele

Zuweisungen

Ergebnis

Seiten / Objekte Layout

Daten / Asset Verknüpfung

Ausgabeformate Print

Web

mobile Endgeräte

RegelwerkeSeitenaufbau / Umbuch

Objektaufbau / Verwendung

Stilvorgaben Absatz

Zeichen

Grep

� Abstimmung /Freigabe

Korrekturworkflow

Statusüberwachung

Remote Publishing / web2Print

� AdaptionFormate

Seiten

Sprachen

Kanal

� ÜbernahmeDaten

Import CSV / XML

http:// Request

ODBC

� ZusammenstellungDokumenten für Ausgabe

� Print

� Web

� mobileEndgeräte

� AktualisierungInhalte

bidirektional

� Konvertierung Seitenformate

JPEG

PDF Print

Web

Office

PNG

� Umsetzung

Seitenaufbau (Print)

Aufbereitung mit Daten

Verknüpfen mit Daten und Assets aus Dateisystem

� Export Inhalte

RTF

XML

� Erstellung TOC / Index / Querverweise

Seitenverweise

Sprungziele Web/URL

Neuer Knoten

� Multimediales Anreichern

SlideShow

Audio / Video Player

Interaktion- Animation - Anwendung- E-Mail / URL- Schaltflächen / Navigation- Formulare

Web-Inhalte

Popup-Windows

scrollbare Bereiche

Reflow-Inhalte (ePub /iPad) oyen.de | 2011

Daten > Layout > Ausgabe

1/9

KW So. 26/08/12

Systemansätze und Überschneidungen

2

KW So. 26/08/12

2/11

Systemansätze und Überschneidungen ...Web Content Management Systemkomplexe Verwaltung von Inhalten auf Internetseiten, kann in Richtung Printausgabe erweitert werden

Printproduktionssystemeffektive Erstellung von Printprodukten mittels Erweiterungen in Desktop Publishing Programmen, lösen sich von Print-orientierung

Media Asset Managementsystem(Universelle) Verwaltung von Bildern, Grafiken und Produktionsdaten. Bieten Workflow- und Publishingfunktionalitäten bis hin zur Printausgabe

KW So. 26/08/12

flexibles Layout ./. automatisiertes Layout

3

KW So. 26/08/12

3/13

flexibles Layout ./. automatisiertes LayoutText um Bild schreibenMedienneutrale und redundanzfreie Speicherung der Inhalte erfolgt im Nachgang

Ausgabe auf KnopfdruckFokus liegt auf Verwaltung, Finden von Informationen, sichern von Fakten und Mehrfachverwendung von (Teil)inhalten.

KW So. 26/08/12

InDesign Doppelseite

autom. Mitteilung / Statusaktion aus InDesign

- Dokumentensperre- Teilsperren für Preise etc.- Verzeichnisüberwachung

05 Layout Status

1. Anmerkungen auf Layoutvorschau (Bild JPEG/PDF) von a) Layout b) Text c) Bild

-> unabhängig von InDesign erstellen -> einblenden und abarbeiten erfolgt in InDesign

2. Speichern in WIS3. Freigaben

01 Annotierung

a) Artikeltextvariantenb) Verwendungc) Übergabe Manuskript Urtext / Änderungen Rücklesen

02 Texte

für D-A-CHa) Preiseb) Landesspezifische Layoutelemente

-> Befüllung per Workflow-> Übergabe Korrekte Best-Nr. Preis, Maße, etc.

03 Platzhalter

a) Top / Top-five (Vignetten) / Serienbilderc) Logosd) Stimmungsbilder

-> Bilder für Litho sammeln-> Palette Briefing Bilder

04 Bilder Pull

WIS 2.x System

- Seitenplanung a) Übernahme b) Neugestaltung c) neue Angebote

- Briefings a) Layout b) Text c) Bild d) Angebote e) Zusammen- stellungen

- Abstimmungen- Korrekturen

- Verwendung- Bereitstellung

Push

3/14

KW So. 26/08/12

Chapter 1-6

P ein Produkt pro Seite

01

Cover / Umschlag

Rücken

Cover

01

Rückseite

02

Vorwort

03

Rückseite

04

Vorlaufseiten (Grau I) römisch nummeriert

01

new

products

02

product literature & support material

03

TOC all chapters

04

List of Intern. Represent

01 Chapter

Num &

Section

index (TOC

small)

02

SectionIntro

04

SonderSeiten

Anhang (Grau II) arabisch nummeriert

01

Trade-

marks

02

Sonder-seiten

03

Patent & LicenceDisclaimer

04

Product Index A-Z

06

Product Index 0-9

07

Terms & Conditions

Faxanforderung

6

103

Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.

Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate

truncated proteins.

Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.

Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).

Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com

BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de

LightCycler 480 SystemInstrument

Rea

l-Ti

me

PC

R S

yste

ms

Rea

l-Ti

me

PC

R

Cat. No. Pack Size Price03 728 790 001 1 set 214,50

(96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).

Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all

regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and

all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade

Additional ProductsProduct 5088, see page 123

6

103

Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.

Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate

truncated proteins.

Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.

Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).

Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com

BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de

LightCycler 480 SystemInstrument

Rea

l-Ti

me

PC

R S

yste

ms

Rea

l-Ti

me

PC

R

Cat. No. Pack Size Price03 728 790 001 1 set 214,50

(96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).

Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all

regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and

all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade

Additional ProductsProduct 5088, see page 123

P Produkt über 2 Seiten

02

6

103

Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.

Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate

truncated proteins.

Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.

Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).

Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com

BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de

LightCycler 480 SystemInstrument

Rea

l-Ti

me

PC

R S

yste

ms

Rea

l-Ti

me

PC

R

Cat. No. Pack Size Price03 728 790 001 1 set 214,50

(96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).

Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all

regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and

all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade

Additional ProductsProduct 5088, see page 123

6

103

Product 4711ApplicationThe RTS Wheat Germ Linear Template Generation Set, His6-tag can be used to rapidly generate linear expression constructs by PCR, ready for use as DNA templates in RTS 100 Wheat Germ CECF and RTS 500 Wheat Germ CECF protein expression reactions. The use of PCR instead of subcloning into vectors allows rapid protein expression from wild-type or modified DNA sequences. This method of generating DNA templates for protein ex-pression avoids the time-consuming step of cloning into expression plasmids and can be used for initial, rapid screening for expression.

Benefits■ Rapidly create linear expression constructs.■ Optimize regulatory elements.■ Save time; no need for laborious cloning.■ Take advantage of flexible positioning of His6-tag.■ Benefit by adding epitope-tag sequences for detection with antibodies.■ Flexibly introduce desired mutations, change codon usage and generate

truncated proteins.

Product DescriptionIn order to generate linear expression constructs, two PCRs must be per-formed. In the first reaction, gene-specific primers are used to add overlap regions to the sequence of the target gene. The product of the first PCR is mixed with two flanking primers and DNA fragments coding for the T7 pro-motor, eukaryotic 5’- and 3’-regulatory elements and a His6-tag sequence (C-terminal or N-terminal). In the second overlap extension PCR, the prod-uct of the first PCR anneals with the added DNA fragments and the 5’ and 3’ ends are extended. This linear expression construct is then amplified via the flanking primers.

Background InformationOne of the major advantages of cell-free compared to cell-based expression systems is the ability to use PCR-generated templates. Using the RTS Wheat Germ Linear Template Generation Set, His6-tag, if primers are designed for the first (gene specific) of the two PCRsaccording to the guidelines provided in the pack insert, the resulting product can be used in a second PCR to pro-duce a completely functional linear expression template for expression in either the RTS 100 E. coli HY Kit (using RTS E. coli Linear Template Genera-tion Sets) or the RTS 100 Wheat Germ CECF Kit (using the RTS Wheat Germ Linear Template Generation Set). Primers for the second PCR are provided in the RTS Wheat Germ Linear Template Generation Set, His6-tag. The de-sign of these kits enables easy transfer of products from any expression sys-tem to both the RTS Wheat Germ and E. coli platforms, as well as between the two RTS systems. At a later step in the RTS workflow, insertion of these products into an RTS pIVEX Wheat Germ vector may be required (e.g., to produce a template for expression scale-up to RTS 500 Wheat Germ CECF, or to ensure that a well-characterized [sequenced] and stabilized template is used in a series of subsequent experiments).

Ordering and product information, or technical support:www.pharma-enhance-science.comSubscriptions for electronic Pharmaica, product spotlights and more:www.pharma-enhance-science.com/enewsInformation on products available in bulk: scbio.pharma.com

BestellungenGebührenfrei: 0800/235-3152Fax: 0621/235-3136Online Shop: www.IBuyPharma.de

LightCycler 480 SystemInstrument

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Cat. No. Pack Size Price03 728 790 001 1 set 214,50

(96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

03 728 790 001 1 set 214,50 (96 reactions)

Figure 1: Principle of the generation of PCR products for in vitro expression using RTS Linear Template Generation Sets. The introduction of an N-terminal His6-tag is shown. For detailed information on the mechanism, refer to the RTS website (www.proteinexpression.com → RTS Wheat Germ Products → Linear Templates and Vectors for both N- and C-terminal tagging).

Contents1. Forward Primer2. Reverse Primer3. C-terminal His6-tag DNA, coding for a C-terminal His6-tag and all

regulatory elements necessary for in vitro protein expression4. N-terminal His6-tag DNA, coding for an N-terminal His6-tag and

all regulatory elements necessary for in vitro protein expression5. Water, PCR-grade

Additional ProductsProduct 5088, see page 123

P mehre Produkte pro Seite

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To place an order: 0800 262 1640 orwww.roche-applied-science.com

Technical inquiries: 800 262 4911e-mail: biochems.us@roche.com

Restriction Enzymes

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Asp EI

Asp EI

Asp EI

Asp EI

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

4

326

To place an order: 0800 262 1640 orwww.roche-applied-science.com

Technical inquiries: 800 262 4911e-mail: biochems.us@roche.com

Restriction Enzymes

Mol

ecul

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iolo

gyM

appi

ng a

nd C

loni

ng o

f Nuc

leic

Aci

ds

Asp EI

Asp EI

Asp EI

Asp EI

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 0%.Pwo SuperYield DNA Polymerase# PCR Mix: 85%; if supplementedwith GC-RICH Solution: 100%. #not available in U.S.Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

from Methanococcus aeolicus PL-15/H

Compatible Ends: None. Isoschizomers: Tth111 I.Methylation Sensitivity: No information available.Activity in PCR buffer: 100%Temperature Optimum: +37°CIsoschizomers: Tth111 I.Heat inactivation: No information available.

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

Cat. No. Pack Size Price 11 131 354 001 400 U 64,50

(10 U/µl)

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

ContentsEnzyme solution supplied with SuRE/Cut Buffer BRestriction Site: GACN↓NNGTC

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

BufferA B L M H50 to 75% 100% 25 to 50% 75 to 100% 75 to 100%

P Kreuztabelle Doppelseite

04

Publishing System

Publishing System generate InDesign pages

Smile

Indesign Book

03

Productpages(4 Vari.)

Heiler Print

Manager

CMS

PublicationsManager

3/15

KW So. 26/08/12

3/15

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Komponenten

4

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4/18

Komponenten .Editorial SystemErstellung und Bearbeitung von Content

-Verwaltungswerkzeuge für Zugriffsunterstützung, Aufgabenverteilung und Statusüberwachung

-Verarbeitungswerkzeuge für Contenterzeugung, properitär und/oder integration externen (Text)Editoren

-> Layout-orientiert -> Zeilenfallverbindlich

-> Content-orientiert -> strukturelle inhaltliche Aufbereitung

KW So. 26/08/12

4/19

Komponenten ..Content RepositoryZentrale Speicherung der erstellten Inhalte

-Trennung von Inhalt und Form -> Multi Format Publishing

-Abbildung Makrostrukturen -> Inhaltebausteine referenzieren

-Abbildung Mikrostrukturen -> Textkörper zerlegen

-gleichzeitiger Zugriff verschiedener Benutzer auf Inhalte

-> Check-Out -> Ein Benutzer bearbeitet Inhalt, Inhalt ist für andere Benutzer gesperrt

-> Check-In -> neue Version wird geladen und zur Bearbeitung durch andere Benutzer freigeben

KW So. 26/08/12

4/20

Komponenten ...Publishing SystemAusgabeseite des Content Management Systems

-Printprodukte -> Seitenvorbereitung -> Ausschiessen -> Farbseparierung

-Onlineprodukte-> Granularität der Gestaltung -> Template / StyleSheet / Skript

-> Zeitpunkt der Erzeugung -> statisch oder dynamisch

KW So. 26/08/12

Zusammenarbeit

5

KW So. 26/08/12

1. Planung & Steuerung

Publishing Prozess 3M mit jCatalog PIM & Cross Media PublisherPreislisten / Katalog

2. Kreation

3. Repro

4. Bilddatenmanagement Datenhandling

Vorlagen-erstellung

5. Reinzeichnung

PDF Druckdaten

Layout-abstimmung

Publikations-planung

BildbearbeitungBilderstellung

Bilder / Texte CSV Daten InDesign.-PDF Dokumente

Seitenaufbau Text / Bild Integration

Aktualisierung Abstimmung

FinishingInh.verz. / TOC

Dokumenten-sammlung

Lektorat

PIM

Cross Media Publisher

Daten

Bilder Texte

oyen.de | 2011

CMS

PublicationsManager

5/22

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ZusammenarbeitTermin- und AblaufplanungBasis für strukturierte Prozesse und Abläufe -> Eskalationsstufen, Benachrichtigung und Aufgabendelegierung

EchtzeitkommunikationInstant Messaging und Chat

WhiteboardingSkizzieren von Anordnungen, Seitenaufbauten

AnnotierungenErgänzend zu Annotierungen im PDF, webasierte, kollaborative Anmerkungen

KW So. 26/08/12

Danke.

KW So. 26/08/12